Structural Biology and Research

Crystallography, NMR & Mass Spectrometry

Nuclear Magnetic Resonance (NMR) spectroscopy has become an indispensable tool in the characterization of structure and dynamics of biological macromolecules such as proteins. In recent years, NMR spectroscopy has contributed to our understanding of protein biophysics, especially when considering time-averaged dynamical events spanning various time scales. It has also played an important role in structural genomics and protein structure initiatives. The main focus of this chapter is to introduce the NMR phenomenon by providing a broad description of NMR spectroscopy and its contribution to the characterization of structure and dynamics of proteins.