Patrick Carl
Bruker Biospin, Germany
Biography
Electron Paramagnetic Resonance (EPR) is a powerful technique used to explore biological macromolecular structures.
Using intrinsic or extrinsic EPR species, the specifi cs of structural topology, protein-protein/protein-RNA interactions and
structural rearrangements are observed. Continuous wave EPR is used to probe the local environment to obtain information
such as pH, viscosity, rotational correlation time, and hydrophobicity. Th is information can easily be obtained using a user
friendly benchtop spectrometer, the EMXnano. Pulse EPR opens the possibility to probe more specifi cally the interactions in
the biological structures. Double Electron Electron Resonance (DEER) permits the direct distance determination from 2 nm
up to 10 nm. Th e DEER technique is not size limited and aides in the determination of multidomain protein and nucleic acid
structures.
Abstract
Abstract : Probing structures, interactions and rearrangements by EPR