Peter B Stathopulos
University of Western Ontario, Canada
Title: Structural elements of stromal interaction molecule mediated store operated calcium entry regulation
Biography
Biography: Peter B Stathopulos
Abstract
Calcium (Ca2+) is a universal signaling entity in eukaryotic cells mediating diverse processes such as the immune response,
hypertrophy, apoptosis, platelet aggregation and memory, to name a few. Th ese processes require a sustained elevation
of cytosolic Ca2+ levels which is facilitated by store operated Ca2+ entry (SOCE). SOCE is the process whereby endoplasmic
reticulum (ER) luminal Ca2+ depletion signals the opening of ion channels on the plasma membrane (PM) which facilitate
the movement of Ca2+ down the concentration gradient from the extracellular space into the cytosol. Th e principal molecules
that mediate SOCE include the ER resident stromal interaction molecule-1 (STIM1) and PM ORAI1 protein subunits which
assemble into a channel pore. Upon ER luminal Ca2+ depletion, STIM1 undergoes a destabilization coupled oligomerization
which leads to translocation of this Ca2+ sensor to ER-PM junctions where it couples to ORAI1 subunits and opens these PM
Ca2+ channels. Since the identifi cation of STIM1 and ORAI1 as the principal molecules driving SOCE, considerable progress
has been made elucidating their high-resolution structural mechanisms of action. Author will present available structural data
on the STIM1 Ca2+ sensing mechanism and how this regulator may complex to ORAI1 subunits. Th e coupling mechanism
revealed using soluble human STIM1 and ORAI1 fragments are congruent with the hexameric assembly elucidated in the D.
melanogaster crystal structure. Finally, author will present unpublished work showing how post translational modifi cations
within the luminal domain of STIM1 aff ects the structural mechanisms of Ca2+ sensing. Ultimately, the post-translation
modifi cation driven STIM1 structural and biophysical changes have implications in the agonist induced hypertrophic response
and pinpoint a new therapeutic target for heart disease.