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Yuri L. Lyubchenko

University of Nebraska Medical Center, USA

Title: Nanoscale dynamics of protein-DNA complexes as revealed with high-speed AFM

Biography

Biography: Yuri L. Lyubchenko

Abstract

High-speed atomic force microscopy (HS-AFM) is an advanced technique with numerous applications in biology,
particularly in molecular biophysics. Developed as a time-lapse AFM technique for direct imaging fully hydrated
biological molecules, HS-AFM is currently capable of visualizing the dynamics of biological molecules and their complexes
at a video-data acquisition rate. Spatial resolution at the nanometer level is another important characteristic of HS-AFM. Th is
presentation focuses on examples of primarily protein-DNA complexes to illustrate the high temporal and spatial resolution
capabilities of HS-AFM that have resulted in novel models and/or the functional mechanisms of these biological systems.