Kazuhiro Ogata
Yokohama City University Graduate School of Medicine, Japan
Title: Molecular behavior of a higher-order complex of multiple transcription factors on enhancer site upon phosphorylation
Biography
Biography: Kazuhiro Ogata
Abstract
Transcriptional regulation is a fundamental mechanism for cell proliferation and differentiation. A major player in this process is a member of tanscription factors (TFs). Activities of TFs are modulated by chemical modifications such as phosphorylation through cell signaling pathways directing cell functions. Molecular mechanisms of regulation for the functional high-order assembly consisting of multiple TFs and enhancer DNA (enhanceosome) under cell signaling are largely unknown. So far, we investigated changes in assembly state of an enhanceosome upon a TF’s phosphorylation, using crystallographical, biophysical, and molecular dynamical analyses. Here, we will illustrate the case of Ets1 in non-phosphorylated and phosphorylated forms on its target gene enhancers with or without its partner TFs, Runx1/CBF or Pax5, as an example. A structure-function relationship of a TF-DNA assembly and an effect by phosphorylation will be discussed.
